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CAL-148

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  • Cat.No.
  • CSC-C0489
  • Description
  • Established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)
  • Recommended Medium
  • 80% DMEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 μg/100 ml)
  • Organism
  • Human
  • Morphology
  • epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype
  • Quality Control
  • Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast adenocarcinoma

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com