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C57BL/6 Mouse Intestinal Smooth Muscle Cells

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Cat.No.
CSC-C4287X
Description
Mouse Intestinal Smooth Muscle Cells from Creative Bioarray are isolated from tissue of pathogen-free laboratory mice. Mouse Intestinal Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.
Mouse Intestinal Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.
Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray.
Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Species
Mouse
Source
Intestine
Recommended Medium
Complete Smooth Muscle Cell Medium
Cell Type
Smooth Muscle
Disease
Normal
Storage and Shipping
Creative Bioarray will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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