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C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells

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  • Cat.No.
  • CSC-C4296X
  • Description
  • Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.
    Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
    Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.
    Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
  • Recommended Medium
  • Complete Mesenchymal Stem Cell Medium
  • Organism
  • Mouse
  • Storage and Shipping
  • Creative Bioarray will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 ?C) until ready for experimental use. Live-cell shipment is also available on request.

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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com