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C-433

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  • Specification
  • Questions & FAQ
  • Recommended Products
  • Cat.No.
  • CSC-C0367
  • Description
  • Thought to be "established from the benign giant cell tumor excised from a woman who had swelling and pain in the right ankle (distal tibia)"; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of cell line RD-ES
  • Organism
  • Human
  • Morphology
  • cells grow loosely adherent in monolayers; some cells grow as round or polygonal cells in clumps in suspension with cellular processes
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypertetraploid karyotype with 4% polyploidy - 103(92-106)<4n>XX, der(X), -X, +1, +1, +1, +1, +2, +6, +6, -14, +17, +18, +6mar, add(X)(p22), del(1)(p22), del(1)(p32p35)x1, der(1)t(1;1)(p32;q11), der(8)t(4;8)(q11;q24), t(9;12)(p12;q13)x2,
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Ewing's sarcoma (derivative of RD-ES)

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com