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  • Cat.No.
  • CSC-C0334
  • Description
  • These cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epitheloid cells growing adherently in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid karyotype with 13% polyploidy - 72(68-74)<3n>XX, -Y, +1, -4, +5, +5, +5, -11, +12, +12, -19, -21, -22, +3mar, der(1)t(1;3)(p11;q11), der(1)t(1;5) (p21;q21), der(2)t(2;5)(p11;q11), del(3)(q13), add(4)(q35), del(5)(p12), i(5p)x3, add(6)(
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endot
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • cervix carcinoma (derivative of HELA)

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com