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  • Cat.No.
  • CSC-C8211L
  • Tissue of Origin
  • Breast
  • Description
  • The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The established population was polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium.
  • Recommended Medium
  • DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine, and 10% FBS
  • Organism
  • Homo sapiens, human
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X, X
    CSF1PO: 10, 12
    D13S317: 11
    D16S539: 8
    D5S818: 11
    D7S820: 9, 10
    THO1: 9,3
    TPOX: 8
    vWA: 15
    D3S1358: 18
    D21S11: 32.2
    D18S51: 15
    Penta E: 14
    Penta D: 13
    D8S1179: 14, 16
    FGA: 19
  • Culture Properties
  • Adherent
  • Karyotype
  • modal number = 78; range = 73 to 80.
    The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:).
  • Source
  • Breast/Mammary
  • Quality Control
  • Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
    specific PCR: negative
  • Storage and Shipping
  • Liquid nitrogen vapor phase
  • Tissue
  • Breast/Mammary
  • Application
  • This cell line can be used as a suitable transfection host.
  • Disease
  • Invasive ductal carcinoma


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