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BT-474

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  • Cat.No.
  • CSC-C0053
  • Description
  • The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus.
  • Recommended Medium
  • DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine, 5µg/ml Insulin and 5% fetal bovine serum.
  • Organism
  • Homo sapiens (human)
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 10,11
    D13S317: 11
    D16S539: 9,11
    D5S818: 11,13
    D7S820: 9,12
    THO1: 7
    TPOX: 8
    vWA: 15,16
    D3S1358: 17
    D21S11: 28,32.2
    D18S51: 13,18
    Penta E: 5
    Penta D: 9,14
    D8S1179: 10,12
    FGA: 22,25
  • Karyotype
  • mode = 55; range = 50 to 112; bimodal shift 58 - 59 and 100 in later passages with 3 marker chromosomes
  • Source
  • Breast
  • Quality Control
  • Plasmotest: negative; Mycoplasma specific PCR: negative
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Breast

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com