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BC-3C

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  • Cat.No.
  • CSC-C0482
  • Description
  • Derived from the invasive solid transitional cell carcinoma of the bladder of a 82-year-old Caucasian woman (grade IV, stage C); cell growth described as being inhibited by EGF
  • Recommended Medium
  • 90% McCoys 5a Medium + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epitheloid adherent cells growing adherently in monolayers and in multilayer foci
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypertriploid karyotype with 10% polyploidy - 65-72<3n>XX, -X, -2, -4, +9, +9, -11, -13, +14, +16, -17, -17, -18, +19, +20, +20, +2-3mar, add(2)(p1?), del(3)(q2?2), der(5;7)(q10;q10), del(9)(p2?2p2?3), add(12)(q24) - sideline with del(2)(
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder transitional cell carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com