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ActoFactor™ Recombinant Rat Placental growth factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0863
  • Description
  • Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse and rat tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Rat PlGF shares 60% amino acid identity with human PlGF2. The gene for PlGF has been mapped to mouse chromosome 12, human chromosome 14 and rat chromosome 6. PlGF binds with highaffinity to Flt1, but not to Flk1/KDR. The full ORF of rat PLGF (Met1-Leu158) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. The native protein expressed in insect cells starts with Ala24. The recombinant rat PLGF-2 is a 15.14 kDa protein containing 135 amino acid residues.
  • Species
  • Rat
  • Product Overview
  • Rat Pgf expressed in Insect cells
  • Molecular Mass
  • N/A
  • Size
  • CAT# CSC-CTK0863-5 (5 μg); CAT# CSC-CTK0863-25 (25 μg)
  • Expression System
  • Insect cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant rat PLGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.1 - 5 ng/mL.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a steril-filtered protein solution in 25 mM Tris (pH 8.5).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com