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ActoFactor™ Recombinant Rat Interleukin 5

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0819
  • Description
  • Interleukin-5 (IL-5), also known as eosinophil differentiation factor (EDF), is a lineage-specific cytokine for eosinophilpoiesis. It regulates eosinophil growth and activation, and thus plays an important role in diseases associated with increased levels of eosinophils, including asthma. IL-5 has a similar overall fold to other cytokines (e.g., IL2, IL4 and GCSF), but while these exist as monomeric structures, IL-5 is a homodimer. The fold contains an anti-parallel 4-alpha-helix bundle with a left handed twist, connected by a 2-stranded anti-parallel beta-sheet . The monomers are held together by 2 interchain disulphide bonds. Recombinant rat IL-5 produced in E. coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13,074 Dalton.
  • Species
  • Rat
  • Product Overview
  • Rat Il5 expressed in E.coli
  • Molecular Mass
  • 13 kDa
  • Size
  • CAT# CSC-CTK0819-10 (10 μg); CAT# CSC-CTK0819-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant rat IL-5 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of BCL-1 cells was found to be < 0.15 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-5 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com