- IL-17E also called IL-25 is a disulfide-linked homodimer of two 145 amino acid polypeptide chains. It belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17E stimulates secretion of IL-8, and induces activation of the transcription factor NK-kB in cells that express the IL-17BR receptor (IL17E and IL17B are ligands for the cytokine receptor IL17BR). IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn't induce allergic inflammation in vivo. Recombinant rat IL-17E produced in E. coli is disulfide-linked homodimer of two 145 amino acid polypeptide chains having a molecular mass of 35.5 kDa. It has been purified by using proprietary chromatographic techniques.
- Product Overview
- Rat Il25 expressed in E.coli
- CAT# CSC-CTK0829-25 (25 μg); CAT# CSC-CTK0829-125 (125 μg)
- Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized (freeze-dried), sterile powder. Lyophilized from a concentrated (1 mg/ml) protein solution with no additives.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-17E should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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