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ActoFactor™ Recombinant Rat Interleukin 17E (IL-25, SF20)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0829
  • Description
  • IL-17E also called IL-25 is a disulfide-linked homodimer of two 145 amino acid polypeptide chains. It belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17E stimulates secretion of IL-8, and induces activation of the transcription factor NK-kB in cells that express the IL-17BR receptor (IL17E and IL17B are ligands for the cytokine receptor IL17BR). IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn't induce allergic inflammation in vivo. Recombinant rat IL-17E produced in E. coli is disulfide-linked homodimer of two 145 amino acid polypeptide chains having a molecular mass of 35.5 kDa. It has been purified by using proprietary chromatographic techniques.
  • Species
  • Rat
  • Product Overview
  • Rat Il25 expressed in E.coli
  • Molecular Mass
  • 35.5 kDa
  • Size
  • CAT# CSC-CTK0829-25 (25 μg); CAT# CSC-CTK0829-125 (125 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • N/A
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized (freeze-dried), sterile powder. Lyophilized from a concentrated (1 mg/ml) protein solution with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-17E should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com