Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Rat Interleukin 1 alpha

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0813
  • Description
  • A soluble factor produced by monocytes, macrophages, and other cells which activates T- lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens and antigens. In human skin fibroblasts, IL1-alpha and TNF-alpha induce the synthesis of IL1-beta. In pheochromocytoma cells, NGF induces the synthesis of IL1. Recombinant rat IL-1alpa produced in E. coli is a single, non-glycosylated polypeptide chain of 155 amino acids and a molecular mass of 17,703 Dalton.
  • Species
  • Rat
  • Product Overview
  • Rat Il1a expressed in E.coli
  • Molecular Mass
  • 17.7 kDa
  • Size
  • CAT# CSC-CTK0813-10 (10 μg); CAT# CSC-CTK0813-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant rat IL-1 alpha is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a specific activity of 2 x 10^8 IU/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50 mM Tris-HCl (pH 8).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-1 alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com