- A soluble factor produced by monocytes, macrophages, and other cells which activates T- lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens and antigens. In human skin fibroblasts, IL1-alpha and TNF-alpha induce the synthesis of IL1-beta. In pheochromocytoma cells, NGF induces the synthesis of IL1. Recombinant rat IL-1alpa produced in E. coli is a single, non-glycosylated polypeptide chain of 155 amino acids and a molecular mass of 17,703 Dalton.
- Product Overview
- Rat Il1a expressed in E.coli
- CAT# CSC-CTK0813-10 (10 μg); CAT# CSC-CTK0813-50 (50 μg)
- Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
- Recombinant rat IL-1 alpha is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a specific activity of 2 x 10^8 IU/mg.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile-filtered white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50 mM Tris-HCl (pH 8).
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-1 alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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