- Recombinant Rat Insulin Like Growth Factor-I (rRa IGF-I) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like and mitogenic activities. This growth factor has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. It stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. Recombinant rat IGF-1 produced in E. coli is a single, non-glycosylated polypeptide chain of 70 amino acids and a molecular mass of 7,687 Dalton.
- Product Overview
- Rat Igf1 expressed in E.coli
- CAT# CSC-CTK0812-50 (50 μg); CAT# CSC-CTK0812-250 (250 μg)
- Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
- Rat IGF-I is fully biologically active when compared to standards. The ED50 was calculated by two different methods: 1. Stimulation of protein synthesis in rat L6 myoblasts results in an ED50 that was less then 30 ng/ml. 2. Type 1 IGF receptor binding assay results in an ED50 Greater than 10 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile-filtered white lyophilized (freeze-dried) pow der. Rat IGF-I was lyophilized from a protein solution with no additives.
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IGF-I should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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