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ActoFactor™ Recombinant Rat Insulin-like growth factor 1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0812
  • Description
  • Recombinant Rat Insulin Like Growth Factor-I (rRa IGF-I) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like and mitogenic activities. This growth factor has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. It stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. Recombinant rat IGF-1 produced in E. coli is a single, non-glycosylated polypeptide chain of 70 amino acids and a molecular mass of 7,687 Dalton.
  • Species
  • Rat
  • Product Overview
  • Rat Igf1 expressed in E.coli
  • Molecular Mass
  • 7.69 kDa
  • Size
  • CAT# CSC-CTK0812-50 (50 μg); CAT# CSC-CTK0812-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Rat IGF-I is fully biologically active when compared to standards. The ED50 was calculated by two different methods: 1. Stimulation of protein synthesis in rat L6 myoblasts results in an ED50 that was less then 30 ng/ml. 2. Type 1 IGF receptor binding assay results in an ED50 Greater than 10 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered white lyophilized (freeze-dried) pow der. Rat IGF-I was lyophilized from a protein solution with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IGF-I should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com