- Receptor-associated Protein (aka RAP) averts the GCM-mediated enhancement of axon growth- ApoE-containing lipoproteins which are known to bind to receptors of the LDLr superfamily. In the presence of RAP, GCM doesn't increase the axon extension rate in relation to base medium. Furthermore, the addition of RAP to the base medium doesn't change the rate of axon extension. This implies that the growth stimulatory effect of apoE-containing lipoproteins secreted by glial cells is mediated by the LDLr family receptors. RAP annuls the growth stimulatory effect of GCM. Recombinant rat RAP produced in E. coli is a single, non-glycosylated polypeptide chain with a molecular mass of 38.86 kDa. It has been purified by proprietary chromatographic techniques.
- Product Overview
- Rat FTO expressed in E.coli
- CAT# CSC-CTK0838-20 (20 μg); CAT# CSC-CTK0838-100 (100 μg)
- Greater than 95% as determined by SDS-PAGE and HPLC analysis.
- Endotoxin Level
- Less than 1 EU/μg.
- White (freeze-dried) powder. Lyophilized from a sterile-filtered, concentrated protein solution in TBS (pH 7.5), 0.1% BSA and 0.09% NaN3.
- Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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