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ActoFactor™ Recombinant Rat Defensin beta 4

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0851
  • Description
  • Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta- defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta- defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta- Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-3 has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Recombinant rat BD-4 produced in E. coli is a single, non-glycosylated polypeptide chain of 41 amino acids and a molecular mass of 4.4 kDa. It has been purified by proprietary chromatographic techniques.
  • Species
  • Rat
  • Product Overview
  • Rat Defb4 expressed in E.coli
  • Molecular Mass
  • 4.4 kDa
  • Size
  • CAT# CSC-CTK0851-20 (20 μg); CAT# CSC-CTK0851-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Recombinant rat BD-3 is fully biologically active when compared to a standard. The biological activity was determined by its antimicrobial activity against E. coli. The ED50 for this effect is typically 5-50 μg/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • White (freeze-dried) powder. Lyophilized from a sterile-filtered (0.2 μm) concentrated protein solution in 1×PBS (pH 7.4).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted BD-4 should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com