- Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL-2 beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant rat GM-CSF produced in E. coli is a single, non-glycosylated polypeptide chain of 128 amino acids and a molecular mass of 14,59 Dalton.
- Product Overview
- Rat CSF2 expressed in E.coli
- CAT# CSC-CTK0807-20 (20 μg); CAT# CSC-CTK0807-100 (100 μg)
- Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
- Determined by its ability to stimulate the proliferation of mouse FDC-P1 cells. The expected ED50 is ≤0.01 ng/ml, corresponding to a specific activity of Greater than1 x 10^8 units/mg.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile-filtered through a 0.2 micron filter and lyophilized from 10mM Tris (pH 8.5).
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa GM-CSF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term, storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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