- M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 18.1 kDa homodimeric protein containing two 155 amino acid polypeptide subunits.
- Product Overview
- Rat Csf1 expressed in E.coli
- CAT# CSC-CTK0809-10 (10 μg); CAT# CSC-CTK0809-50 (50 μg)
- Greater than 98% as determined by SDS-PAGE and HPLC analysis.
- The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M- NFS-60 cells is ≤ 5.0 ng/ml, corresponding to a specific activity of Greater than 2 x 10^5 units/mg.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered solution in 5 mM sodium phosphate (pH 7.5).
- Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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