- MDC (CCL22) is a small cytokine that belongs to the CC chemokine family. CCL22 is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. MDC shows chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. On the other hand, MDC shows a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. MDC may also have a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. MDC interacts with cell surface chemokine receptors CCR4. CCL22 is vastly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. CCL22 is also found in the lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression of CCL22 can be seen in the lung and the spleen and very weak expression in the small intestine. In the lymph node CCL22 is expressed in a mature subset of Langerhans cells (CD1a+ and CD83+). Furthermore, CCL22 is expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. In addition, MDC has a role in hindering progression of lung cancer. Moreover, significantly higher CCL22 expression is linked to gastric cancer. Recombinant rat MDC is a 7.9 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines. It has been purified by proprietary chromatographic techniques.
- Product Overview
- Rat Ccl22 expressed in E.coli
- CAT# CSC-CTK0872-20 (20 μg); CAT# CSC-CTK0872-100 (100 μg)
- Greater than 97% as determined by SDS-PAGE and HPLC analysis.
- Fully biologically active when compared to standard. Biological activity was determined by its ability to chemoattract human activated lymphocytes using a concentration range of 10.0-100.0 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- White (freeze-dried) powder. Lyophilized from a sterile-filtered (0.2 μm), concentrated protein solution in 1×PBS, (pH 7.4).
- Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at –20°C. Reconstituted MDC should be used immediately or stored in undiluted working aliquots at –20°C. Avoid repeated freeze / thaw cycles.
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