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ActoFactor™ Recombinant Mouse Vascular endothelial cell growth factor 120

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0720
  • Description
  • VEGF-120 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF-120 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1). Consistent with the endothelial cell-specific action of VEGF-120, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen, it is a potent vascular permeability factor (VPF) in vivo and is also a chemo attractant for monocytes and endothelial cells. At least four different proteins are generated by differential splicing of the mouse VEGF gene: VEGF-120, VEGF-144, VEGF-164 and VEGF-188. The most abundant form is VEGF-164. Whereas VEGF-120, VEGF-144 and VEGF-164 are secreted proteins, VEGF-188 is strongly cell-associated. In addition, the isoforms VEGF-164 and VEGF-188 bind to heparin with high affinity. VEGF is apparently a homodimer, but preparations of VEGF show some heterogeneity on SDS gels depending of the secretion of different forms and the varying degrees of glycosylation. All dimeric forms possess similar biological activities. There is evidence that heterodimeric molecules between the different isoforms exists and that different cells and tissues express different VEGF isoforms. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. Mouse Vascular Endothelial Growth Factor 120 (VEGF-120), a 14.1 kDa protein consisting of 120 amino acid residues, is produced as a homodimer (28.2 kDa).
  • Species
  • Mouse
  • Product Overview
  • Mouse Vegfa expressed in E.coli
  • Molecular Mass
  • 14.1 kDa
  • Size
  • CAT# CSC-CTK0720-5 (5 μg); CAT# CSC-CTK0720-25 (25 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for VEGF- 120 has been determined to be in the range of 2-10 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered protein solution in 50 mM acetic acid.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 50 mM acetic acid to a concentration not lower than 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com