- RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate na?ve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant human sRANKL is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).
- Product Overview
- Mouse Tnfsf11 expressed in E.coli
- CAT# CSC-CTK0711-10 (10 μg); CAT# CSC-CTK0711-50 (50 μg)
- Greater than 95% as determined by SDS-PAGE and HPLC analysis.
- Determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0-10.0 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered protein solutio n in 10 mM sodium phosphate (pH 7.5) and 50 mM NaCl with no additives.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile-ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted sRANKL should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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