- Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.|Recombinant mouse Prolactin produced in E. coli is a single, non-glycosylated polypeptide chain of 198 amino acids having a molecular mass of 22.5 kDa. Prolactin has been purified by using proprietary chromatographic techniques.
- Product Overview
- Mouse Prl expressed in E.coli
- CAT# CSC-CTK0656-50 (50 μg); CAT# CSC-CTK0656-250 (250 μg)
- Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
- The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma Nb2-11 was found to be < 0.065 ng/ml.
- Lyophilized from a concentrated (1 mg/ml), sterile- filtered protein solution with in 10 mM sodium phosphate buffer (pH 8) and 50 mM Nacl with no additives.
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at –20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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