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ActoFactor™ Recombinant Mouse Platelet derived growth factor, B polypeptide

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0707
  • Description
  • Recombinant Murine Platelet Derived Growth Factor (rMu PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Murine PDGF-BB is a 24.446 kDa homodimeric, non- glycosylated, polypeptide chain of 109 amino acid residues.
  • Species
  • Mouse
  • Product Overview
  • Mouse Pdgfb expressed in E.coli
  • Molecular Mass
  • 24.4 kDa
  • Size
  • CAT# CSC-CTK0707-10 (10 μg); CAT# CSC-CTK0707-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, RP-HPLC and AEX-FPLC analysis.
  • Activity
  • The ED50 has been determined by the dose-dependent stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • The protein was lyophilized from 10 mM NaCitrate (pH 3.0).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized PDGF-BB should be reconstituted in sterile 100 mM acetic acid and 0.1% BSA to a concentration of 0.1- 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized rMu PDGF-BB, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. For long-term storage, reconstituted rMu PDGF-BB should be stored in undiluted working aliquots at ( -20°C. It is also recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com