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ActoFactor™ Recombinant Mouse Leukemia inhibitory factor
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Cat.No.
CSC-CTK0659
Description
LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells. Recombinant mouse LIF produced in plant contains 188 amino acids and a 16 a.a. histidine- based tag for a total length of 204 a.a., and has a predicted molecular mass of 22.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 34 kDa in SDS-PAGE.
Species
Mouse
Product Overview
Mouse Lif expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
22.8 kDa
Size
CAT# CSC-CTK0659-10 (10 μg); CAT# CSC-CTK0659-50 (50 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
Mouse embryonic stem (ES) cells were cultured without MEFs (mouse embryonic fibroblasts) in a conditioned medium using LIF concentrations of 1 ng/ml, 5 ng/ml or 10 ng/ml. All concentration conditions resulted in morphologically normal ES cell colonies. Furthermore, in an additional test, when the ES cells were deprived of LIF they spontaneously differentiated and were able to form characteristic embryoid bodies in hanging drops.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.2).
Reconstitution
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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