- IL-17E (also called IL-25) has a sequence similarity with IL17. It induces NF-kappaB activation, and stimulates the production of IL-8. IL-17E and IL-17B are ligands for the cytokine receptor IL- 17BR. IL-17E is a proinflammatory cytokine favoring Th2-type immune response. The upregulation of costimulation-induced IL-17E receptors and release of cytokines and chemokines from IL-17E treated costimulated Th cells are differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. Blocking IL-17E prevents airway hyperresponsiveness, a critical feature of clinical asthma. IL-17E produced by innate effector eosinophils and basophils increase the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells. Over expression of IL-17E up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. IL-17E contributes to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts which supports the fact that IL-17E is an important factor in asthma pathophysiology. IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn't induce allergic inflammation in vivo. Mouse recombinant IL-17E produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 306 amino acids and having a molecular mass of 34.9 kDa.
- Product Overview
- Mouse Il25 expressed in E.coli
- CAT# CSC-CTK0695-25 (25 μg); CAT# CSC-CTK0695-125 (125 μg)
- Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
- Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution containing no additives.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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