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ActoFactor™ Recombinant Mouse Interleukin 12B

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0690
  • Description
  • Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells. Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits.
  • Species
  • Mouse
  • Product Overview
  • Mouse Il12b expressed in CHO cells
  • Molecular Mass
  • 75 kDa
  • Size
  • CAT# CSC-CTK0690-10 (10 μg); CAT# CSC-CTK0690-50 (50 μg)
  • Expression System
  • CHO cells
  • Purity
  • Greater than 98% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Determined by ist ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤ 0.1 ng / ml, corresponding to a specific activity of Greater than 1 ×10^7 units/mg. The ED50 has also been determined by the dose-dependent stimulation of INF-gamma production by NK cells co-stimulated with IL-18 is ≤ 0.1 ng / ml, corresponding to a specific activity of Greater than 1 ×10^7 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered protein solution in 1 x PBS (pH 7.2).
  • Reconstitution
  • Please centrifuge product before opening vial. Reconstitute in sterile PBS (pH 7.2) to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. This solution can than be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted murine IL-12 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com