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ActoFactor™ Recombinant Mouse Interleukin-10

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0049
  • Description
  • Mouse Interleukin 10 (IL-10) is a species-specific pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, proliferation, immunoglobulin secretion and class II MHC expression. IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. As an immunosuppressant, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by downregulating class II MHC expression. It inhibits IFN-γ synthesis and Th1 differentiation in the mouse system. Recombinant mouse IL-10 has a predicted molecular mass of approximately 18 kDa.
  • Species
  • Mouse
  • Product Overview
  • Mouse Il10 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature mouse IL-10 protein sequence was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0049-5 (5 μg); CAT# CSC-CTK0049-25 (25 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of mouse IL-10 is measured in a cell proliferation assay using the murine mast cell line, MC/9-2. The ED50 for this effect is typically 0.4 - 1.2 ng/mL.
  • Endotoxin Level
  • Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
  • Formulation
  • Recombinant mouse IL-10 is lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 7.0 containing 50 μg bovine serum albumin per 1 μg cytokine.
  • Reconstitution
  • Reconstitute mouse IL-10 at a concentration greater than 1 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
  • Storage & Stability
  • Lyophilized mouse IL-10 is stable at -20°C to -70°C for up to twelve months from date of receipt. Reconstituted mouse IL-10 can be stored under sterile conditions at 2 - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com