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ActoFactor™ Recombinant Mouse Insulin-like growth factor 1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0032
  • Description
  • Insulin-like growth factor-1 (IGF-1) is structurally homologous to proinsulin. IGF-1 is produced by several cell types and may have autocrine, paracrine and endocrine functions. IGF-1 is a potent mitogen that mediates the growth-promoting activities of growth hormone postnatally. It also plays a role during embryonic growth and differentiation. Recombinant mouse IGF-1 is a 7.6 kDa protein containing 70 amino acid residues. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain.
  • Species
  • Mouse
  • Product Overview
  • Mouse Igf1 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature IGF-1 protein was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0032-50 (50 μg); CAT# CSC-CTK0032-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was measured in a serum-free cell proliferation assay using the human cell line MCF-7 and the ED50 was typically 1.0-3.0 ng/ml.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in PBS.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 25 μg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Upon reconstitution, mouse IGF-1 can be stored under sterile conditions at 2° - 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com