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ActoFactor™ Recombinant Mouse Heparin-binding EGF-like growth factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0662
  • Description
  • HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant mouse HB-EGF produced in E. coli is a single, non-glycosylated polypeptide chain of 86 amino acids (63-148 a.a.) and a molecular mass of 9.8 kDa. It has bee purified by proprietary chromatographic techniques
  • Species
  • Mouse
  • Product Overview
  • Mouse Hbegf expressed in E.coli
  • Molecular Mass
  • 9.8 kDa
  • Size
  • CAT# CSC-CTK0662-50 (50 μg); CAT# CSC-CTK0662-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • The ED50, as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤1.0 ng/ml corresponding to a specific activity of Greater than1 x 10^6 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • White (freeze-dried) powder. The protein was lyophi lized from a sterile-filtered, concentrated (1 mg/ml) protein solution in 1xPBS (pH 7.4).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized rMu HB-EGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, should be stored desiccated at -20°C. Reconstituted rMu HB-EGF should be used immediately or stored in undiluted working aliquots at -20°C. Please avoid repeated freeze / thaw cycles. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com