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ActoFactor™ Recombinant Mouse Colony stimulating factor 1 (macrophage)
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Cat.No.
CSC-CTK0671
Description
Recombinant Murine Macrophage Colony Stimulating Factor (rMu M-CSF) is M a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. RMu M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant murine M-CSF is a 36.4 kDa homodimeric protein containing two 156 amino acid polypeptide subunits..
Species
Mouse
Product Overview
Mouse Csf1 expressed in E.coli
Molecular Mass
36.4 kDa
Size
CAT# CSC-CTK0671-10 (10 μg); CAT# CSC-CTK0671-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is typically 0.5-3 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized with 0.5x PBS, pH 8.0.
Reconstitution
Please centrifuge product before opening vial. The lyophilized powder should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized rMu M-CSF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu M-CSF should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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