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ActoFactor™ Recombinant Mouse Chemokine (C-X-C motif) ligand 9

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0747
  • Description
  • CXCL9 is a chemoattractant for activated T cells having angiostatic activity. Both CXCL9 and CXCL10 have been considered as important components for the anti-tumor activities of interferon-gamma (IFN-gamma) and interleukin-12 in animal models. Human MIG is induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-gamma but not by interferon-alpha or by lipopolysaccharides. Analysis of mouse and human genomic DNAs suggested that the respective human and murine genes are true mouse-human homologues. Recombinant human MIG (monokine induced by gamma-interferon) produced in E. coli is a single, non-glycosylated polypeptide chain of 103 amino acids and a molecular mass of 11,700 Dalton. Recombinant mouse MIG (monokine induced by gamma-interferon) produced in E. coli is a single, non-glycosylated polypeptide chain of 105 amino acids and a molecular mass of 12,208 Dalton.
  • Species
  • Mouse
  • Product Overview
  • Mouse Cxcl9 expressed in E.coli
  • Molecular Mass
  • 12.2 kDa
  • Size
  • CAT# CSC-CTK0747-20 (20 μg); CAT# CSC-CTK0747-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant MIG is fully biologically active when compared to a standard. The biological activity is calculated by its ability to chemoattract human lymphocytes using a concentrations ranging from 0.1-1 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml), sterile solution in water containing no add itives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu MIG should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com