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ActoFactor™ Recombinant Mouse Chemokine (C-X-C motif) ligand 12

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0062
  • Description
  • Stromal cell-derived factor-1 (SDF-1), also known as Pre-B cell growth stimulating factor (PBSF), is an a family (CXC) chemokine secreted by bone marrow, stromal cells, liver tissue and muscle. SDF-1 is chemoattractant for T lymphocytes, monocytes, pro-and pre-B cells but not neutrophils. SDF-1 was shown to be a ligand for fusin/LESTR, a CXCR4 type receptor that functions as a coreceptor for HIV-1 strains. Recently, SDF-1 was shown to induce lymphocyte homing, mature blood cell functional activation and stem cell growth inhibition. SDF-1 is also a chemoattractant for CD34+ haemopoietic progenitors. Mouse SDF-1a is synthesized as a precursor of 89 amino acid residues processed to the mature form by cleavage of the N-terminus. Recombinant mouse SDF-1a contains 68 amino acid residues and has a predicted molecular mass of 8 kDa.
  • Species
  • Mouse
  • Product Overview
  • Mouse Cxcl12 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature mouse SDF-1a protein (aa residues 22-89) was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0062-10 (10 μg); CAT# CSC-CTK0062-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined by the ability to chemoattract cultured human lymphocytes and mouse BaF/3 cells transfected with the human CXCR4 receptor. The ED50 using this assay was typically 3 – 9 ng/ml and 0.15 – 0.6 ng/mL, respectively.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution of 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
  • Storage & Stability
  • The lyophilized sampleis stable for greater than sixmonths at -20°C to -70°C. Reconstituted mouse SDF-1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss ofactivit y. Avoid repeated freezing and thawing.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com