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ActoFactor™ Recombinant Mouse Chemokine (C-C motif) ligand 3

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Cat.No.
CSC-CTK0749
Description
Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ, with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Recombinant murine MIP-1 alpha is a 7.8 kDa protein containing 69 amino acid residues.
Species
Mouse
Product Overview
Mouse Ccl3 expressed in E.coli
Molecular Mass
7.8 kDa
Size
CAT# CSC-CTK0749-10 (10 μg); CAT# CSC-CTK0749-50 (50 μg)
Expression System
E.coli
Purity
Greater than 99% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract murine balb/c splenocytes using a concentration range of 1-10 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The sterile-filtered protein solution was lyophilized with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-1 alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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