- Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ, with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Recombinant murine MIP-1 alpha is a 7.8 kDa protein containing 69 amino acid residues.
- Product Overview
- Mouse Ccl3 expressed in E.coli
- CAT# CSC-CTK0749-10 (10 μg); CAT# CSC-CTK0749-50 (50 μg)
- Greater than 99% as determined by SDS-PAGE and HPLC analysis.
- Determined by its ability to chemoattract murine balb/c splenocytes using a concentration range of 1-10 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- The sterile-filtered protein solution was lyophilized with no additives.
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-1 alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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