- TARC, a CC chemokine, is predominantly produced by dendritic cells in the thymus and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. Recombinant mouse TARC produced in E. coli is an 7.9 kDa protein containing 70 amino acid residues. It has been purified by proprietary chromatographic techniques.
- Product Overview
- Mouse Ccl17 expressed in E.coli
- CAT# CSC-CTK0788-20 (20 μg); CAT# CSC-CTK0788-100 (100 μg)
- Greater than 97% as determined by SDS-PAGE and HPLC analysis.
- Fully biologically active when compared to a standard. The ED50 as determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR4 is typically 2-10 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- White (freeze-dried) powder. Lyophilized from a ste rile-filtered (0.2 μm) concentrated protein solution in 1×PBS (pH 7.4).
- Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted TARC should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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