- Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (adipokine). The protein belongs to the soluble defence collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
- Product Overview
- Mouse Adipoq expressed in HEK cells
- CAT# CSC-CTK0767-10 (10 μg); CAT# CSC-CTK0767-50 (50 μg)
- Expression System
- HEK cells
- Greater than 98% as determined by SDS-PAGE and HPLC analysis.
- White, lyophilized (freeze-dried) powder. Sterile-f iltered and lyophilized from a 0.5 mg/ml protein solution in TBS buffer.
- Please centrifuge product briefly before opening vial. For reconstitution, add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. This solution can then be stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu Acrp30-HEK should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles., cell culture and/or animal studies. Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocytes (Yamauchi T. et al., 2002). It can also activate AMPK in HepG2 human hepatocytes at a concentration of 1.0 μg/ml. Full-length adiponectin purified from mammalian cells can acutely decrease blood glucose levels (Berg A.H. et al., 2001). Single subcutaneous injection of adiponectin at the dose of 30 mg/g body weight significantly decreased the blood glucose levels in C57 mice.
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