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ActoFactor™ Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 2 (sKDR, native; Insect cell)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0411
  • Description
  • Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR -1 (Flt-1), VEGFR -2 (KDR/Flk-1), VEGFR -3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR -1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR -2 has a lower affinity for VEGF than the Flt -1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR -2 leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin. Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 2 (sKDR) generated by alternative splicing consist of the first 6 Ig-like loops followed by the unique C-terminal end: CGRETILDHSAEAVGMP. It contains 659 amino acids and has a molecular weight of 105 kDa (monomer).
  • Species
  • Human
  • Product Overview
  • Human KDR expressed in Insect cells
  • Molecular Mass
  • 105 kDa
  • Size
  • CAT# CSC-CTK0411-5 (5 μg); CAT# CSC-CTK0411-25 (25 μg)
  • Expression System
  • Insect cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • Measured by its ability to bind to immobilized recombinant human VEGF-165 in a functional ELISA.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered protein solution in 25 mM MES, 100 mM NaCl (pH 5.5).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water or PBS to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com