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ActoFactor™ Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-5 (flt-1; Sf9 Insect)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0408
  • Description
  • Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-5 (sVEGFR-1 D1-5) is a 70 kDa protein. The baculovirus generated, recombinant human sVEGFR-1 is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 5 extracellular domains, which contain all the information necessary for high affinity ligand binding. The receptor monomers have a mass of approximately 70 kDa. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
  • Species
  • Human
  • Product Overview
  • Human FLT1 expressed in Insect cells
  • Molecular Mass
  • 70 kDa
  • Size
  • CAT# CSC-CTK0408-5 (5 μg); CAT# CSC-CTK0408-25 (25 μg)
  • Expression System
  • Insect cells
  • Purity
  • Greater than 90% as determined by SDS-PAGE analysis.
  • Activity
  • The activity of sVEGFR-1 (D1-5) was determined by its ability to inhibit the VEGF-A induced proliferation of HUVECs. The ED50 for this effect is typically between 12-30 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein is soluble in water and most aqueous buffers. It should be reconstituted in sterile PBS to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C or -70°C. Reconstituted rHu sVEGFR-1 (D1-5) should be used immediately or stored long-term in undiluted working aliquots at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com