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ActoFactor™ Recombinant Human soluble TIE-2

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0597
  • Description
  • TIE-1 and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)- like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) precursor protein with an 18 aa putative signal peptide, a 727 aa extracellular domain and a 354 aa cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind TIE-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Recombinant human soluble TIE-2/TEK was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Thr19-Lys745). It contains 731 amino acids and has a molecular weight of 95 kDa.
  • Species
  • Human
  • Product Overview
  • Human TEK expressed in Insect cells
  • Molecular Mass
  • 95 kDa
  • Size
  • CAT# CSC-CTK0597-10 (10 μg); CAT# CSC-CTK0597-50 (50 μg)
  • Expression System
  • Insect cells
  • Purity
  • Greater than 90% as determined by SDS-PAGE analysis.
  • Activity
  • Measured in a functional ELISA assay. When sTIE-2-His is immobilized at 4 μg/ml (100 μl/well), it binds recombinant human Angiopoietin-2 with a linear range of 2-100 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a steril-filtered protein solution in PBS without any stabilizers.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in ultra-pure, sterile water or aqueous buffers to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sTIE-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com