- WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP- 1 is a beta catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot- like (CTCK) domain.
- Product Overview
- Human WISP1 expressed in E.coli
- CAT# CSC-CTK0615-20 (20 μg); CAT# CSC-CTK0615-100 (100 μg)
- Greater than 98% as determined by SDS-PAGE and HPLC analysis.
- The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 μg/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered solution with no additives.
- Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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