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ActoFactor™ Recombinant Human Vascular endothelial cell growth factor 121 (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0389
  • Description
  • Recombinant Human Vascular Endothelial Cell Growth Factor121 (rHu VEGF-121) is a 28.4 kDa homodimeric protein consisting of two non-glycosylated 121 amino acid polypeptide chains. VEGF-121 is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF-121 include stimulation of endothelial cell growth, angiogenesis, and capillary permiability. VEGF-121 does not bind on heparin.
  • Species
  • Human
  • Product Overview
  • Human VEGFC expressed in E.coli
  • Molecular Mass
  • 28.4 kDa
  • Size
  • CAT# CSC-CTK0389-10 (10 μg); CAT# CSC-CTK0389-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE.
  • Activity
  • The ED50 as determined by (3H)-thymidine incorporation into human umbilical vein endothelial (HUVE) cells and stimulation of cell proliferation was found to be at a concentration of 0.2-0.4 ng/ml corresponding to a specific activity of 2.5 - 5 x 10^6 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a concentrated protein solution (1 mg/ml) with no additives.
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration not lower than 50 μg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
  • Storage & Stability
  • Lyophilized cytokine is stable as shipped for at least 1 year at 20°C to 70°C. Reconstituted protein should be stored in working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com