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ActoFactor™ Recombinant Human Vascular endothelial cell growth factor 121
- Specification
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Cat.No.
CSC-CTK0390
Description
VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. VEGF-121 has increased acidity which allowes it to circulate more freely than other VEGF forms. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity. Recombinant human VEGF-121 produced in plant contains 110 amino acids and a 16 a.a. histidine-based tag for a total length of 126 a.a., and has a predicted molecular mass of 14.4 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 19 kDa in SDS-PAGE.
Species
Human
Product Overview
Human VEGFC expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
14.4 kDa
Size
CAT# CSC-CTK0390-10 (10 μg); CAT# CSC-CTK0390-50 (50 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
The ED50 is determined by a cell proliferation assay measuring the dose-dependent effects of serial dilutions of human VEGF-121 on HUVEC cells. The ED50 value for VEGF121 lies typically in the range of 2.8-4.3 ng/ml. The optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Sterile filtered through a 0.2 μm filter. Lyophilized from 10 mM acetic acid (pH 5.0).
Reconstitution
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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