- VEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine. It promotes DNA synthesis in endothelial cells, helps regulate angiogenesis and vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons. VEGF-B is expressed in all tissues except the liver. It forms cell surfaced-associated disulfide linked homodimers and can form heterodimers with VEGF-A. There are two known isoforms, formed by alternative splicing, which have been designated VEGF-B167 and VEGF-B186. Both forms have identical amino-terminal sequences encoding a "cysteine knot" like structural motif, but differ in their carboxyl-terminal domains. Both VEGF-B isoforms signal only through the VEGFR1 receptor. Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.
- Product Overview
- Human FLT1 expressed in E.coli
- CAT# CSC-CTK0391-20 (20 μg); CAT# CSC-CTK0391-100 (100 μg)
- Greater than 98% as determined by SDS-PAGE and HPLC analysis.
- Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVECs) in the presence of human VEGF165. The expected ED50 for this effect is 1.0 - 2.0 μg/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from 10 mM Acetic Acid.
- Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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