Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 13b (19 kDa)

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0356
  • Description
  • BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF. Recombinant BAFF has been produced by transient expression of BAFF in non-transgenic plant. It consists of a glycosilated polypeptide chain with 151 amino acids (134-285 aa; Q9Y275 [TN13B_HUMAN]) fused to a 10XHis tag at the N-terminus and has a molecular mass betwen 18 and 20kDa. Recombinant BAFF has been purified by sequential chromatography (affinity and anionic FPLC) and does not contain animal–derived components or impurities.
  • Species
  • Human
  • Product Overview
  • Human TNFSF13B expressed in Nicotiana benthamiana
  • Molecular Mass
  • 18-20 kDa
  • Size
  • CAT# CSC-CTK0356-10 (10 μg); CAT# CSC-CTK0356-50 (50 μg)
  • Expression System
  • Nicotiana benthamiana
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • The biological activity has been determined by the dose-dependent stimulation of the proliferation of B cells from human PBMC. Cell proliferation was measured using the MTT method. The ED50 was found to be ≤50 ng/ml*. *Activity results may vary with PBMC donors.
  • Endotoxin Level
  • Less than 0.04 EU/μg.
  • Formulation
  • Recombinant human BAFF is lyophilized from 20 mM PBS buffer (pH 7.0) and 0.2 M NaCl.
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 25-50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com