ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 11

Specification
Recommended Products

Cat.No.

CSC-CTK0296

Description

Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodelling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signalling complex involving SRC kinase and tumor necrosis factor receptor- associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases. Human sRANKL is expressed in Nicotiana benthamiana is a glycosylated polypeptide chain containing 175 amino acids (70 – 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N- terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosylation, the recombinant protein could migrate as two bands with an apparent molecular mass of 21-23 kDa in SDSPAGE. The recombinant protein is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal- derived components or impurities.

Species

Human

Product Overview

Human TNFSF11 expressed in Nicotiana benthamiana

Molecular Mass

21.1 kDa

Size

CAT# CSC-CTK0296-10 (10 μg); CAT# CSC-CTK0296-50 (50 μg)

Expression System

Nicotiana benthamiana

Purity

Greater than 97% as determined by SDS-PAGE gel analysis.

Activity

No commercial bioassay is currently available. The identity of the protein was confirmed by Western blotting with protein specific antibodies.

Endotoxin Level

Less than 0.04 EU/μg.

Formulation

Recombinant human RANKL is lyophilized from 10 mM phosphate potasium buffer (pH 8.0) and 0.2 M NaCl.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentration the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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