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ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 10

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0334
  • Description
  • Recombinant Human TRAIL (rHu TRAIL, TNF-Related Apoptosis Inducing Ligand), also called Apo2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. Creative Bioarrays Human TRAIL/Apo2 Ligand is a 19.6 kDa protein with 168 amino acid residues, comprising the full-length of the TNF-like extracellular domain of TRAIL/Apo2 Ligand.
  • Species
  • Human
  • Product Overview
  • Human TNFSF10 expressed in E.coli
  • Molecular Mass
  • 19.6 kDa
  • Size
  • CAT# CSC-CTK0334-50 (50 μg); CAT# CSC-CTK0334-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and HPLC analysis.
  • Activity
  • Recombinant Human TRAIL is fully biologically active when compared to a standard. The ED50 as determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells was found to be 2 ng/ml, corresponding to a specific activity of 5 x 10^5 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered and lyophilized from a concentrate d solution (1 mg/ml) containing 150 mM NaCl and 50 mM sodium phosphate (pH 7.4).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.5-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized TRAIL/Apo2L, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted TRAIL/Apo2L should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com