Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Tumor necrosis factor (Yeast)

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0344
  • Description
  • Recombinant Human Tumor Necrosis Factor-alpha (rHu TNF-alpha) is a 17 kDa protein containing 157 amino acid residues. Human TNF-alpha is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
  • Species
  • Human
  • Product Overview
  • Human TNF expressed in Yeast
  • Molecular Mass
  • 17 kDa
  • Size
  • CAT# CSC-CTK0344-50 (50 μg); CAT# CSC-CTK0344-250 (250 μg)
  • Expression System
  • Yeast
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • Biological activity was determined by a standard cell cytotoxicity assay using the TNF-alpha susceptible mouse cell line L929 in the presence of 1 μg/ml Actinomycin D. The ED50 for this effect is ≤0.05 ng/ml, which corresponds to a specific activity of Greater than 2 x 10^7 units/mg (WHO Standard 87/650, NICBS).
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized (Stabilizer: Mannitol 50 μg/μg rHu TNF-alpha).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized rHu TNF- alpha should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% HSA or BSA) should be added to avoid loss of bioactivity.
  • Storage & Stability
  • The lyophilized rHu TNF-alpha, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TNF-alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com