Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Tumor necrosis factor (E. coli)

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0341
  • Description
  • TNF is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 and GM-CSF. Recombinant human Tumor Necrosis Factor-alpha produced in E. coli is a single, non-glycosylated polypeptide chain of 158 amino acids (157 aa of the mature human TNF-alpha and an N-terminal methionine) and a molecular mass of 17,483.77 Dalton.
  • Species
  • Human
  • Product Overview
  • Human TNF expressed in E.coli
  • Molecular Mass
  • 17.48 kDa
  • Size
  • CAT# CSC-CTK0341-50 (50 μg); CAT# CSC-CTK0341-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.05 ng/ml, corresponding to a specific activity of 2 x 10^7 IU/mg.
  • Endotoxin Level
  • N/A
  • Formulation
  • Lyophilized from an sterile-filtered protein soluti on containing 20 mM PBS (pH-7.2) and 100 mM NaCl.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized rHu TNF- alpha should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. For further dilution, carrier protein (0.1% HSA or BSA) should be added to avoid loss of bioactivity.
  • Storage & Stability
  • The lyophilized rHu TNF-alpha, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TNF-alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com