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ActoFactor™ Recombinant Human Tumor necrosis factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0008
  • Description
  • Tumor Necrosis Factor - alpha (TNF-a) occurs as a secreted, soluble form and as a membrane anchored form, both of which are biologically active. TNF-a is a pleiotropic factor activating multiple signal transduction pathways and inducing or suppressing the expression of a wide variety of genes. TNF-a plays a critical role in mediation of the inflammatory response and in influencing resistance to infections and tumor growth. Recombinat human TNF-a is a 17.5 kDa protein containing 158 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human TNF expressed in E.coli
  • Sequence
  • A DNA sequence encoding mature human TNF-a was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0008-10 (10 μg); CAT# CSC-CTK0008-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of recombinant TNF-a was measured in a cytotoxicity assay using the TNF-susceptible L-929 cell line in the presence of the metabolic inhibitor, actinomycin D. The ED50 for this effect is typically 0.02 - 0.05 ng/mL.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
  • Storage & Stability
  • The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted TNF-a can be stored under sterile conditions at 2°C to 4°C for one month or at -20 °C to -70 °C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com