- TGFBR2 is part of the Ser/Thr protein kinase family and the TGFB receptor subfamily. TGFBR2 is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 expression is increased in oral squamous cell carcinoma cells. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity of tamoxifen metabolites in breast cancer. Human Recombinant TGFBR2 produced in E. coli is a single, non-glycosylated, polypeptide chain encoding 133 amino acids (4-136 aa, the extracellular domain of TGFBR2) with a molecular mass of 15.1 kDa.
- Product Overview
- Human TGFBR2 expressed in E.coli
- CAT# CSC-CTK0333-10 (10 μg); CAT# CSC-CTK0333-50 (50 μg)
- Greater than 97% as determined by SDS-PAGE analysis.
- Measured by its ability to inhibit TGF-b1 activity on a mouse T cell line, HT-2.
- Lyophilized from a sterile-filtered solution in buffer containing 150 mM NaCl and 50 mM sodium phosphate buffer (pH 7.5).
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa Leptin should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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