Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Transforming growth factor, beta 3

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0330
  • Description
  • Transforming growth factor-betas (TGF-betas) are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor-beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues. Transforming Growth Factor-beta 3 (TGF-beta 3) is a new isoform of the TGF-beta superfamily and is presumed to play an important role in wound repair and scarring. TGF-beta 3 is believed to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis. Recombinant human TGF-beta 3 produced by transient expression of TGF-β3 in non-transgenic plants contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product does not contain any animal-derived components or impurities. Recombinant human TGF-β3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond.
  • Species
  • Human
  • Product Overview
  • Human TGFB3 expressed in Nicotiana benthamiana
  • Molecular Mass
  • 27.2 kDa
  • Size
  • CAT# CSC-CTK0330-5 (5 μg); CAT# CSC-CTK0330-25 (25 μg)
  • Expression System
  • Nicotiana benthamiana
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • The biological activity of TGF-β3 was measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. The ED50 was found to be ≤50 ng/ml.
  • Endotoxin Level
  • Less than 0.04 EU/μg.
  • Formulation
  • Lyophilized from a 0.05 M Tris-HCl buffer (pH 7.4).
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 5-25 ng/μl. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com