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ActoFactor™ Recombinant Human Transforming growth factor, beta 1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0059
  • Description
  • Human Transforming Growth Factor-β1 (TGF-β1) is a pleiotropic cytokine produced primarily by platelets and bone, and in smaller amounts by most nucleated cell types and tumors. TGF-β1 is involved in tissue remodelling, wound repair, development and hematopoiesis. TGF-β1 is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is also a switch factor for IgA. Human TGF-β1 is synthesized as a dimeric inactive precursor of 390 amino acid residues, which is cleaved to an active dimer of 112 residues disulphide-linked peptides. The mature protein has a molecular mass of 25 kDa. TGF-β1 binds to three types of receptors. Type I and Type II TGF-β1 receptors encode serine/threonine kinases and mediate signal transduction events via autophosphorylation.
  • Species
  • Human
  • Product Overview
  • Human TGFB1 expressed in CHO cells
  • Sequence
  • A DNA sequence encoding the human TGF-β1 precursor protein was expressed in CHO cells. Mature human TGF-β1 was purified from the acidified conditioned media.
  • Size
  • CAT# CSC-CTK0059-2 (2 μg); CAT# CSC-CTK0059-10 (10 μg)
  • Expression System
  • CHO cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of recombinant human TGF-β1 was measured by its inhibition of the murine IL-4-dependent H-thymidine incorporation by HT-2 cells. The ED50 for this inhibition was typically 0.04 - 0.2 ng/mL.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile plus 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of the cytokine.
  • Reconstitution
  • Purified recombinant human TGF-β1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, lyophilized samples should be reconstituted with sterile 4 mM HCl containing 1 mg/mL bovine serum albumin or human serum albumin to a final TGF-β1 concentration of no less than 1 μg/mL.
  • Storage & Stability
  • Lyophilized samples are stable for greater than 6 months at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2-4°C for 1 month or at -20°C to -70°C for 3 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com