- Human Transforming Growth Factor-β1 (TGF-β1) is a pleiotropic cytokine produced primarily by platelets and bone, and in smaller amounts by most nucleated cell types and tumors. TGF-β1 is involved in tissue remodelling, wound repair, development and hematopoiesis. TGF-β1 is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is also a switch factor for IgA. Human TGF-β1 is synthesized as a dimeric inactive
precursor of 390 amino acid residues, which is cleaved to an active dimer of 112 residues disulphide-linked peptides. The mature protein has a molecular mass of 25 kDa. TGF-β1 binds to three types of receptors. Type I and Type II TGF-β1 receptors encode serine/threonine kinases and mediate signal transduction events via autophosphorylation.
- Product Overview
- Human TGFB1 expressed in CHO cells
- A DNA sequence encoding the human TGF-β1 precursor protein was expressed in CHO cells. Mature human TGF-β1 was purified from the acidified conditioned media.
- CAT# CSC-CTK0059-2 (2 μg); CAT# CSC-CTK0059-10 (10 μg)
- Expression System
- CHO cells
- Greater than 97% as determined by SDS-PAGE and visualized by silver stain
- The biological activity of recombinant human TGF-β1 was measured by its inhibition of the murine IL-4-dependent H-thymidine incorporation by HT-2 cells. The ED50 for this inhibition was typically 0.04 - 0.2 ng/mL.
- Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
- Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile plus 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of the cytokine.
- Purified recombinant human TGF-β1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, lyophilized samples should be reconstituted with sterile 4 mM HCl containing 1 mg/mL bovine serum albumin or human serum albumin to a final TGF-β1 concentration of no less than 1 μg/mL.
- Storage & Stability
- Lyophilized samples are stable for greater than 6 months at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2-4°C for 1 month or at -20°C to -70°C for 3 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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